H (0) blood group determinant is present on soluble human L-selectin expressed in BHK-cells

AbstractIn the present study we show that the H (0) blood group determinant Fucα1-2Galβ1-4GlcNAcβ1-R is present on N-linked glycans of soluble human L-selectin recombinantly expressed in baby hamster kidney (BHK) cells. The glycans were isolated using complementary HPLC techniques and characterized by a combination of exoglycosidase digestion and mass spectrometry. The linkage of the fucose residues was determined by incubation of the glycans with specific fucosidases. The H blood determinant Fucα1-2Galβ1-4GlcNAcβ1 was detected for bi-, 2,4 branched tri- and tetraantennary structures. To our knowledge, the proposed oligosaccharide structures represent a new glycosylation motif for recombinant glycoproteins expressed on BHK cells