Association of eNOS (E298D) and CYP2J2 (−50G/T) gene polymorphisms with hypertension among Egyptian cases

AbstractBackgroundHypertension is a multifactorial and polygenic disorder where several susceptible genes interact with the environmental factors.ObjectiveThis study was designed to investigate the frequency of the endothelial nitric oxide synthase (eNOS, E298D) and cytochrome P450 2J2 (CYP 2J2 −50G/T) gene polymorphisms among Egyptian hypertensive subjects.Subjects and methodsGenomic DNA was isolated from peripheral blood samples collected from 123 hypertensive cases and from 429 healthy non-related controls by using Magna pure system (Roche Molecular Biochemicals, Mannheim, Germany). DNA was used to determine the frequency of eNOS, E298D and CYP 2J2 −50G/T allelic variants using the Light Cycler polymerase chain reaction (Realtime PCR) technique. Genotype (wild type, mutant and heterozygous) was determined by melting curve analysis.ResultsRegarding the study of eNOS and CYP2J2 gene polymorphisms, analysis of total hypertensive cases exhibited higher frequency of mutant eNOS heterozygous ED genotype compared to controls, yet statistically insignificant (27.6% vs. 21.1%, OR=1.5, 95% CI=0.9–2.8, p=0.1). Similarly, analysis of hypertensive cases also exhibited higher frequency of mutant CYP2J2 −50GT genotypes compared to controls that was also statistically insignificant (13.8% vs. 10.7%, OR=1.3, 95% CI=0.7–2.4, p=0.4). All subgroups of hypertensive complicated cases either with cardiac diseases, with diabetic or with renal disorders showed insignificant difference compared to controls.ConclusionThe present study demonstrated no association between eNOS E298D and CYP2J2 −50 G/T gene polymorphisms and hypertension among Egyptian subjects