Identification of the 3′ - and 5′ -ends of beet necrotic yellow vein virus RNAs

AbstractBeet necrotic yellow vein virus RNAs are characterized by a 5′ -end cap structure and polyadenylated 3′ -ends. The presence of a poly(A) sequence with major lengths ranging from 65–140 nucleotides could be demonstrated by homochromatography and sequencing methods. Beet necrotic yellow vein virus RNAs could be 5′ -end labelled with kinase only after enzymatic or chemical cap elimination followed by dephosphorylation. The 5′ -end structure is found to be of cap O type: m7GpppNp