6-Fluoropyridoxol: A novel probe of cellular pH using 19F NMR spectroscopy
- Mehta, Vimal D.
- Kulkarni, Padmakar V.
- Mason, Ralph P.
- Constantinescu, Anca
- Aravind, Sivasubramanian
- Goomer, Naresh
- Antich, Peter P.
DOIAbstract
Abstract6-Fluoropyridoxol was evaluated as an intracellular pH indicator. This molecule exhibits exceptional sensitivity to changes in pH (∼ 10 ppm acid/base shift) and a pKa ∼8.2 appropriate for physiological investigations. Using 19F NMR spectroscopy we determined both intra- and extracellular pH in whole blood and confirmed the measurements using traditional techniques: ion-electrodes and 31P NMR spectroscopy
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