Refined Genetic Mapping of the Darier Locus to a <1-cM Region of Chromosome 12q24.1, and Construction of a Complete, High-Resolution P1 Artificial Chromosome/Bacterial Artificial Chromosome Contig of the Critical Region
- Monk, Sarah
- Sakuntabhai, Anavaj
- Carter, Simon A.
- Bryce, Steven D.
- Cox, Roger
- Harrington, Louise
- Levy, Elaine
- Ruiz-Perez, Victor L.
- Katsantoni, Eleni
- Kodvawala, Ahmer
- Munro, Colin S.
- Burge, Susan
- Larrègue, Marc
- Nagy, Gyula
- Rees, Jonathan L.
- Lathrop, Mark
- Monaco, Anthony P.
- Strachan, Tom
- Hovnanian, Alain
DOIAbstract
SummaryDarier disease (DD) (MIM 124200) is an autosomal dominant skin disorder characterized by loss of adhesion between epidermal cells and by abnormal keratinization. We present linkage analysis showing, in four families, key recombination events that refine the location of the DD locus on chromosome 12q23-24.1 to a region of <1 cM. We have constructed a YAC/P1 artificial chromosome (PAC)/bacterial artificial chromosome (BAC)–based physical map that encompasses this refined DD region. The map consists of 35 YAC, 69 PAC, 16 BAC, and 2 cosmid clones that were ordered by mapping 54 anonymous sequence-tagged sites. The critical region is estimated to be 2.4 Mb in size, with an average marker resolution of 37.5 kb. The refinement of the critic...
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