The release of a 10-kDa polypeptide from everted photosystem II thylakoid membranes by alkaline tris

AbstractThe release of polypeptides from inside-out thylakoid vesicles and photosystem II by alkaline Tris treatment was reinvestigated, using SDS-polyacrylanude gel electrophoresis (PAGE) in the presence of urea, with highly increased resolution in the low molecular mass region. In addition to the 33-, 23-, and 16-kDa proteins of the oxygen-evolving complex, a 10-kDa polypeptide was released. This 10-kDa polypeptide is an entrinsic polypeptide located at the inner grana thylakoid surface and with a likely role in photosynthetic oxygen evolution