A functional Spo0A is required for maximal aprE expression in Bacillus subtilis

AbstractThe initiation of sporulation in Bacillus subtilis is under control of the transcriptional factor SpoOA. Most SpoOA mutants fail to initiate the sporulation process and all the sporulation initiated processes such as the synthesis of subtilisin. However, the product of spoOA9V, one of the several spoOA mutants characterized, distinguishes itself in the fact that, while it appears to effectively repress abrB, it fails to activate the spoIIA operon. The aim of this study was to examine the effect of the spoOA9V mutation on aprE expression and we found that in different genetic backgrounds, the spoOA9V mutation has a negative effect on aprE::lacZ expression