Differential Osmotic Behavior of Water Components in Living Skeletal Muscle Resolved by 1H-NMR
- Kimura, Masako
- Takemori, Shigeru
- Yamaguchi, Maki
- Umazume, Yoshiki
DOIAbstract
AbstractUsing frog sartorius muscle, we observed transverse relaxation processes of 1H-NMR signals from myowater. The process could be well described by four characteristic exponentials: the extremely slow exponential of relaxation time constant T2>0.4s, the slow one of T2∼ 0.15s, the intermediate one of 0.03 s<T2<0.06s, and the rapid one of T2<0.03s. Addition of isotonic extracellular solution affected only the extremely slow exponential, linearly increasing its amplitude and gradually increasing its T2 toward that of the bulk solution (1.7s). Therefore, this exponential should represent extracellular surplus solution independently of the other exponentials. At two thirds to three times the isotonicity, the amplitude of the intermediate ex...
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