Cultured epithelial autografts are often applied to wounds with a capacity for regeneration from denial appendages. It is unclear in these circumstances whether the cultured autografts act merely as a biologic dressing or whether they become incorporated into the new epithelium. We have used retroviral gene transfer techniques to identify autologous keratinocytes in an established porcine model of cultured epidermal (CE) grafting. Porcine keratinocytes were transduced with an MFG-lacZ nls vector produced by the amphotropic packaging line GP+EnvAm12. Transduction rates of 15.1%, in the absence of selection, were achieved by a single passage on γ-irradiated retroviral producers as a feeder layer. Full-thickness wounds were created on Large Wh...
Few data are available on early regeneration of human epidermis in vivo. We have established a supra...
In a search for the optimal nonviral gene transfer technique in epidermal and dermal supportive extr...
Definitive treatment to achieve wound healing in major burns frequently include skin transplantation...
Cultured epithelial autografts are often applied to wounds with a capacity for regeneration from den...
Direct transfer of new genetic information to keratinocytes in epidermis may prove effective in trea...
Currently, the use of cultured epithelial autografts as an alternative to split-thickness skin autog...
Introduction: Banked cultured Langerhans cell (LC) free allogeneic keratinocytes, could be a readily...
The combination of cultured autologous keratinocytes with the dermal regeneration template Integra™ ...
Epidermal growth factor (EGF), which promotes epidermal regeneration and wound closure, is important...
An improved method of in vitro cultivation of porcine keratinocytes by which keratinocyte sheets sui...
SIGLEAvailable from British Library Document Supply Centre-DSC:DXN005066 / BLDSC - British Library D...
Background: Extended full thickness skin defects still represent a considerable therapeutic challeng...
Retrovirus-mediated gene transfer is an efficient means of introducing and expressing exogenous gene...
Background. Autologous dermal fibroblasts may be useful in the treatment of skin wounds and for the ...
Background: A large number of different wound coverings have been used in order to improve the wound...
Few data are available on early regeneration of human epidermis in vivo. We have established a supra...
In a search for the optimal nonviral gene transfer technique in epidermal and dermal supportive extr...
Definitive treatment to achieve wound healing in major burns frequently include skin transplantation...
Cultured epithelial autografts are often applied to wounds with a capacity for regeneration from den...
Direct transfer of new genetic information to keratinocytes in epidermis may prove effective in trea...
Currently, the use of cultured epithelial autografts as an alternative to split-thickness skin autog...
Introduction: Banked cultured Langerhans cell (LC) free allogeneic keratinocytes, could be a readily...
The combination of cultured autologous keratinocytes with the dermal regeneration template Integra™ ...
Epidermal growth factor (EGF), which promotes epidermal regeneration and wound closure, is important...
An improved method of in vitro cultivation of porcine keratinocytes by which keratinocyte sheets sui...
SIGLEAvailable from British Library Document Supply Centre-DSC:DXN005066 / BLDSC - British Library D...
Background: Extended full thickness skin defects still represent a considerable therapeutic challeng...
Retrovirus-mediated gene transfer is an efficient means of introducing and expressing exogenous gene...
Background. Autologous dermal fibroblasts may be useful in the treatment of skin wounds and for the ...
Background: A large number of different wound coverings have been used in order to improve the wound...
Few data are available on early regeneration of human epidermis in vivo. We have established a supra...
In a search for the optimal nonviral gene transfer technique in epidermal and dermal supportive extr...
Definitive treatment to achieve wound healing in major burns frequently include skin transplantation...