The Effect of S-Layer Protein Adsorption and Crystallization on the Collective Motion of a Planar Lipid Bilayer Studied by Dynamic Light Scattering
- Hirn, Rainer
- Schuster, Bernhard
- Sleytr, Uwe B.
- Bayerl, Thomas M.
DOIAbstract
AbstractA dedicated dynamic light scattering (DLS) setup was employed to study the undulations of freely suspended planar lipid bilayers, the so-called black lipid membranes (BLM), over a previously inaccessible spread of frequencies (relaxation times ranging from 10−2 to 10−6s) and wavevectors (250cm−1<q<38,000cm−1). For a BLM consisting of 1,2-dielaidoyl-sn-3-glycero-phosphocholine (DEPC) doped with two different proportions of the cationic lipid analog dioctadecyl-dimethylammonium bromide (DODAB) we observed an increase of the lateral tension of the membrane with the DODAB concentration. The experimentally determined dispersion behavior of the transverse shear mode was in excellent agreement with the theoretical predictions of a first-or...
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