Background: One of the main distinctions between different theories describing protein folding is the predicted sequence of secondary structure formation and compaction during the folding process. Whether secondary structure formation precedes compaction of the protein molecules or secondary structure formation is driven by a hydrophobic collapse cannot be decided unequivocally on the basis of existing experimental data.Results: In this study, we investigate the refolding of chemically denatured, disulfide-intact ribonuclease A (RNase A) by monitoring compaction and secondary structure formation using stopped-flow dynamic light scattering and stopped-flow CD, respectively. Our data reveal the formation of a considerable amount of secondary ...
A key problem in experimental protein folding is that of characterizing the conformational ensemble ...
AbstractWhat is the first step in protein folding – hydrophobic collapse (compaction) or secondary s...
We used force-measuring optical tweezers to induce complete mechanical unfolding and refolding of in...
BACKGROUND: One of the main distinctions between different theories describing protein folding is th...
Background: One of the main distinctions between different theories describing protein folding is th...
The folding pathway of Escherichia coli RNase H is one of the best experimentally characterized for ...
The number of distinct protein folding pathways starting from an unfolded ensemble, and hence, the f...
The health of a cell depends on accurate translation and proper protein folding, whereas misfolding ...
The dimensions and secondary structure content of two proteins which fold in a two-state manner are ...
Proteins can sample a variety of partially folded conformations during the transition between the un...
A recently developed method is used here to characterize some of the folding intermediates, and the ...
Using small-angle X-ray scattering combined with a continuous-flow mixing device, we monitored the m...
Ribonuclease A (RNase A), an unusually well defined enzyme, has been a test protein in the study of ...
Background: Comprehension of the rules that govern the folding process is still far from satisfactor...
The conformational folding of the nativelike intermediate des-[40-95] on the major oxidative folding...
A key problem in experimental protein folding is that of characterizing the conformational ensemble ...
AbstractWhat is the first step in protein folding – hydrophobic collapse (compaction) or secondary s...
We used force-measuring optical tweezers to induce complete mechanical unfolding and refolding of in...
BACKGROUND: One of the main distinctions between different theories describing protein folding is th...
Background: One of the main distinctions between different theories describing protein folding is th...
The folding pathway of Escherichia coli RNase H is one of the best experimentally characterized for ...
The number of distinct protein folding pathways starting from an unfolded ensemble, and hence, the f...
The health of a cell depends on accurate translation and proper protein folding, whereas misfolding ...
The dimensions and secondary structure content of two proteins which fold in a two-state manner are ...
Proteins can sample a variety of partially folded conformations during the transition between the un...
A recently developed method is used here to characterize some of the folding intermediates, and the ...
Using small-angle X-ray scattering combined with a continuous-flow mixing device, we monitored the m...
Ribonuclease A (RNase A), an unusually well defined enzyme, has been a test protein in the study of ...
Background: Comprehension of the rules that govern the folding process is still far from satisfactor...
The conformational folding of the nativelike intermediate des-[40-95] on the major oxidative folding...
A key problem in experimental protein folding is that of characterizing the conformational ensemble ...
AbstractWhat is the first step in protein folding – hydrophobic collapse (compaction) or secondary s...
We used force-measuring optical tweezers to induce complete mechanical unfolding and refolding of in...