Protein Architecture of the Human Kinetochore Microtubule Attachment Site
- Wan, Xiaohu
- O'Quinn, Ryan P.
- Pierce, Heather L.
- Joglekar, Ajit P.
- Gall, Walt E.
- DeLuca, Jennifer G.
- Carroll, Christopher W.
- Liu, Song-Tao
- Yen, Tim J.
- McEwen, Bruce F.
- Stukenberg, P. Todd
- Desai, Arshad
- Salmon, E.D.
DOIAbstract
SummaryChromosome segregation requires assembly of kinetochores on centromeric chromatin to mediate interactions with spindle microtubules and control cell-cycle progression. To elucidate the protein architecture of human kinetochores, we developed a two-color fluorescence light microscopy method that measures average label separation, Delta, at <5 nm accuracy. Delta analysis of 16 proteins representing core structural complexes spanning the centromeric chromatin-microtubule interface, when correlated with mechanical states of spindle-attached kinetochores, provided a nanometer-scale map of protein position and mechanical properties of protein linkages. Treatment with taxol, which suppresses microtubule dynamics and activates the spindle ch...
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