New functions for the components of glucocorticoid receptor apparatus?

AbstractBy ammonium sulphate precipitation, phosphocellulose and DEAE—Sephacel chromatography and Sephadex G-75 gel filtration, a factor was separated from rat liver cytosol which was shown to suppress the inhibitory effect of the steroid deoxycorticosterone (DOC) on the specific [3H]glucocorticoid binding to cytoplasmic receptors. By SDS-polyacrylamide gel electrophoresis (SDS—PAGE) of factor-containing fractions its Mr was suggested to be about 40000. The possible role of this factor in the regulation of glucocorticoid receptor apparatus function is discussed