Very rapid clearance after a joint bleed in the canine knee cannot prevent adverse effects on cartilage and synovial tissue
- Jansen, N.W.D.
- Roosendaal, G.
- Wenting, M.J.G.
- Bijlsma, J.W.J.
- Theobald, M.
- Hazewinkel, H.A.W.
- Lafeber, F.P.J.G.
DOIAbstract
SummaryObjectiveJoint bleeding leads to joint destruction. In vitro exposure of human and canine cartilage to blood results in long-lasting severe adverse changes in cartilage. An in vivo joint haemorrhage in the canine knee joint demonstrates similar adverse effects although significantly less outspoken. As a possible explanation for this discrepancy, we studied the clearance rate of blood from the canine knee joints.MethodsBlood was injected into the knee joint of Beagle dogs either 48h, 24h or 15min before termination. The amount of red blood cells (RBC) and white blood cells (WBCs) present in the joint cavity was determined. Chondrocyte activity and cartilage matrix integrity as well as cartilage destructive activity of synovial tissue ...