Anti-Acetylcholinesterase Activity of Piper sarmentosum by a Continuous Immobilized-enzyme Assay

AbstractRecent promising enzymatic methods are gaining attention for the screening of safe inhibitors. The physiologically significant enzymes can be obtained from natural resources used for clinical and pharmaceutical purposes. Piper sarmentosum is reported to have antioxidant activity. Its use in folk medicines suggests that it contains anti-acetylcholinesterase compounds, which have not been discovered yet. In this study we have used flow injection analysis (FIA) technique by immobilizing the enzyme in a column for AChE assay on the crude extract of plant for four different solvents. The results obtained in this research shows that ethanolic extract has strong inhibition (IC50= 128ug/L), whereas methanol extract has medium inhibition (IC50= 127 ug/L) while Ethyle acetate and water has weaker inhibitory activity (IC50= 317 ug/L and IC50= 348 ug/L). From the results, it is suggested that piper sarmentosum have the ability to be a potential inhibitor for the development of anti-Alzheimer disease treatment