Combined transplantation of endothelial progenitor cells and mesenchymal stem cells into a rat model of isoproterenol-induced myocardial injury

SummaryBackgroundEndothelial progenitor cells (EPCs) and mesenchymal stem cells (MSCs) have different biological properties, but their potential for synergy in the treatment of injured myocardium has not been studied extensively.AimTo determine if outcome could be improved by simultaneously transplanting MSCs and EPCs into a rat model of isoproterenol (ISO)-induced injured myocardium.MethodsFour weeks after ISO injection, 50 rats were separated randomly into five groups (n=10 per group) and allocated to receive a saline injection (control group), 200μL medium alone, 200μL medium plus 2×106 EPCs, 200μL medium plus 2×106 MSCs, or 200μL medium plus a combination of 1×106 EPCs and 1×106 MSCs. Echocardiography and invasive catheterization were performed to evaluate dynamic changes in cardiac performance, 12 weeks after treatment administration.ResultsTransplanted cells were detected in myocardial tissue by fluorescence in situ hybridization, indicating either differentiation or integration into cardiac tissue cells. The group of rats that received both EPCs and MSCs had an increased level of angiogenic growth factors expression, less collagen deposition, fewer apoptotic cells and an improved regional myocardial blood flow compared with the other groups; these effects resulted in greater enhancement of cardiac function in that group.ConclusionTransplantation of EPCs combined with MSCs may represent a novel and efficient therapeutic strategy for enhancing regional myocardial blood flow and improving cardiac function in injured myocardium