Synchronization of nuclear DNA synthesis in cultured Daucus carota L. cells by aphidicolin

AbstractBy inhibiting the α-like DNA polymerase, and therefore nuclear DNA synthesis, aphidicolin induces accumulation of suspension cultured carrot cells at the G1/S boundary of the cell cycle. After a 24-h treatment with the drug the accumulation is complete, affecting all the cycling cells (95% of the population). Upon removal of the inhibitor, all cycling cells immediately resume nuclear DNA synthesis and move synchronously throughout the S-phase