Identification of the α3-subunit in the GABAA receptor purified from bovine brain

AbstractPolyclonal antibodies have been raised to synthetic amino acid sequences of the bovine GABAA receptor α1 and α3 subunits. Anti-α1 subunit antibodies recognise a polypeptide of 53 kDa whereas anti-α1 subunit antibodies recognise a polypeptide of 59–60 kDa, in Western blots of GABAA receptor purified from adult bovine cerebral cortex, cerebellum and 12-day calf cerebral cortex