Two Supporting Factors Greatly Improve the Efficiency of Human iPSC Generation
- Zhao, Yang
- Yin, Xiaolei
- Qin, Han
- Zhu, Fangfang
- Liu, Haisong
- Yang, Weifeng
- Zhang, Qiang
- Xiang, Chengang
- Hou, Pingping
- Song, Zhihua
- Liu, Yanxia
- Yong, Jun
- Zhang, Pengbo
- Cai, Jun
- Liu, Meng
- Li, Honggang
- Li, Yanqin
- Qu, Xiuxia
- Cui, Kai
- Zhang, Weiqi
- Xiang, Tingting
- Wu, Yetao
- Zhao, Yiding
- Liu, Chun
- Yu, Chen
- Yuan, Kehu
- Lou, Jinning
- Ding, Mingxiao
- Deng, Hongkui
DOIAbstract
SummaryHuman fibroblasts can be induced into pluripotent stem cells (iPSCs), but the reprogramming efficiency is quite low. Here, we screened a panel of candidate factors in the presence of OCT4, SOX2, KLF4, and c-MYC in an effort to improve the reprogramming efficiency from human adult fibroblasts. We found that p53 siRNA and UTF1 enhanced the efficiency of iPSC generation up to 100-fold, even when the oncogene c-MYC was removed from the combinations
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