Enamel and dentine remineralization by nano-hydroxyapatite toothpastes

AbstractObjectivesThis in vitro study evaluated the effects of nano-hydroxyapatite (n-HAp) toothpastes on remineralization of bovine enamel and dentine subsurface lesions.MethodsSpecimens were demineralized, randomly divided into five groups, and exposed to an aqueous remineralizing solution for two and five weeks (37°C). Brushing procedures were performed with the respective toothpaste/storage solution slurry twice daily (2×5s; total contact time of the slurries 2×120s/d): storage in remineralizing solution only (0); additional brushing with B (20wt% zinc carbonate nano-hydroxyapatite, ZnCO3/n-HAp); BS (24wt% ZnCO3/n-HAp); E (0.14wt% amine fluoride); or A (7wt% pure n-HAp). Differences in mineral loss (ΔΔZ) before and after storage/treatment were microradiographically evaluated.ResultsDentine groups 0, B, BS, and A showed significantly higher ΔΔZ values compared to E (p<0.05; ANOVA). Enamel ΔΔZ values of group A were significantly higher compared to group E (p<0.05), whilst no significant differences of these groups could be observed compared to 0, B, and BS (p>0.05).ConclusionsWith the in vitro conditions chosen, toothpastes containing n-HAp revealed higher remineralizing effects compared to amine fluoride toothpastes with bovine dentine, and comparable trends were obtained for enamel