AbstractPhospholipase C activity against phosphoinositides in isolated rat liver plasma membranes has been examined using exogenous substrates. The enzyme hydrolyzed phosphatidylinositol 4,5-bisphosphate 30–40-times faster than phosphatidylinositol 4-monophosphate, while phosphatidylinositol was not a substrate. Maximum activity was observed with 1.1 mM phosphatidylinositol 4,5-bisphosphate at pH 5.0. The enzyme was stimulated by micromolar concentrations of Ca2+. The GTP analogue guanylyl (β,γ-methylene)diphosphonate enhanced phospholipase C activity at and above 0.3 μM Ca2+, but was inhibitory at 0.1 μM Ca2+. This supports the suggestion that plasma membrane phospholipase C is regulated by guanine nucleotide-binding protein, but indicates...
AbstractVasopressin stimulates the liberation of labelled inositol phosphate in partially purified p...
AbstractVarious adenine nucleotides activated phospholipase C of FRTL-5 cell membranes in the follow...
We previously reported (Ryu, S. H., Cho, K. S., Lee, K. Y., Suh, P. G., and Rhee, S. G. (1986) Bioch...
AbstractPhospholipase C activity against phosphoinositides in isolated rat liver plasma membranes ha...
AbstractHormonal regulation of phospholipase D (PLD) was studied in isolated rat liver plasma membra...
AbstractRat liver plasma membranes are enriched in a Ca2+-dependent phosphodiesterase active on phos...
1. 1. Rat liver plasma membranes were isolated by rate-isopycnic zonal centrifugation. A method is d...
The regulation of soluble phosphoinositide-specific phospholipase C from adult human epidermis by gu...
AbstractPhosphoinositide-specific phospholipase C (PLC) activity of human platelet membranes was act...
AbstractWith [14C]oleate-labeled phosphatidylcholine as a substrate for phospholipase D the hydrolyt...
Hydrolysis of polyphosphoinositides by phospholipase C was examined in isolated membranes prepared f...
AbstractThe treatment of rat liver plasma membranes with EDTA resulted in substantial release of a p...
AbstractLow concentrations of GTP (10–50 μM) greatly enhance the inositol 1,4,5-trisphosphate stimul...
AbstractLipocortins I and II, known to inhibit phospholipase A2, have been purified from bovine lung...
AbstractPhospholipase C (PLC) was purified from the membrane-depleted rat liver nuclei. About 60% of...
AbstractVasopressin stimulates the liberation of labelled inositol phosphate in partially purified p...
AbstractVarious adenine nucleotides activated phospholipase C of FRTL-5 cell membranes in the follow...
We previously reported (Ryu, S. H., Cho, K. S., Lee, K. Y., Suh, P. G., and Rhee, S. G. (1986) Bioch...
AbstractPhospholipase C activity against phosphoinositides in isolated rat liver plasma membranes ha...
AbstractHormonal regulation of phospholipase D (PLD) was studied in isolated rat liver plasma membra...
AbstractRat liver plasma membranes are enriched in a Ca2+-dependent phosphodiesterase active on phos...
1. 1. Rat liver plasma membranes were isolated by rate-isopycnic zonal centrifugation. A method is d...
The regulation of soluble phosphoinositide-specific phospholipase C from adult human epidermis by gu...
AbstractPhosphoinositide-specific phospholipase C (PLC) activity of human platelet membranes was act...
AbstractWith [14C]oleate-labeled phosphatidylcholine as a substrate for phospholipase D the hydrolyt...
Hydrolysis of polyphosphoinositides by phospholipase C was examined in isolated membranes prepared f...
AbstractThe treatment of rat liver plasma membranes with EDTA resulted in substantial release of a p...
AbstractLow concentrations of GTP (10–50 μM) greatly enhance the inositol 1,4,5-trisphosphate stimul...
AbstractLipocortins I and II, known to inhibit phospholipase A2, have been purified from bovine lung...
AbstractPhospholipase C (PLC) was purified from the membrane-depleted rat liver nuclei. About 60% of...
AbstractVasopressin stimulates the liberation of labelled inositol phosphate in partially purified p...
AbstractVarious adenine nucleotides activated phospholipase C of FRTL-5 cell membranes in the follow...
We previously reported (Ryu, S. H., Cho, K. S., Lee, K. Y., Suh, P. G., and Rhee, S. G. (1986) Bioch...