Phosphofructokinase (Pfk) Regulation of Glycolysis in Psoriatic Epidermis

The regulation of glycolysis by phosphofructokinase (PFK) was studied in psoriatic epidermis by measuring the acute changes in the levels of adenosine triphosphate, glucose-6-phosphate plus fructose-6-phosphate, fructose-1,6-diphosphate, and lactate after periods of ischemia. In all experiments, acute ischemia caused decreases in the levels of adenosine triphosphate, glucose, and glucuse-6-phosphate plus fructose-6-phosphate, and increases in the levels of fructose-1,6-diphosphate and lactate. The rapid depletion of glycolytic intermediates before PFK and the accumulation of those after the enzyme indicate activation of the enzyme by ischemia in psoriatic epidermis