Pre-transplantation assessment of renal viability with 31P magnetic resonance spectroscopy

Pre-transplantation assessment of renal viability with 31P magnetic resonance spectroscopy. As acute tubular necrosis (ATN) is still an important cause for postoperative malfunction of renal grafts, it would be useful to have a method predicting such a complication. We investigated the possibility to predict ATN by measuring the ratio of phosphomonoesters (PME, largely consisting of adenosine monophosphate) and inorganic phosphate (Pi) in the renal tissue, using 31P magnetic resonance spectroscopy (MRS) during the cold ischemia period. Assuming that this ratio reflects the tissue high-energy phosphate status, we studied five kidneys from living related donors (LRD), 28 kidneys from heart beating donors (HBD) and nine kidneys from non-heart beating donors (non-HBD). All kidneys were preserved with a phosphate free solution. We found an inverse relation between the time of 31P MRS and the PME/Pi ratio, suggesting a graded decay of tissue high energy phosphates during cold ischemia. The PME/Pi ratio was highest in grafts from LRD (2.65 ± 0.50, no ATN), intermediate in grafts from HBD (1.65 ± 0.41, 21% ATN) and lowest in those derived from non-HBD (1.05 ± 0.47, 56% ATN). The differences in PME/Pi ratio between the groups was statistically significant (P < 0.01). Moreover, the ratio was significantly lower in grafts developing ATN (1.73 ± 0.41 vs. 1.35 ± 0.29 in the HBD group, 1.41 ± 0.24 vs. 0.76 ± 0.36 in the non-HBD group, P < 0.05). These observations point to a general relation between the pre-transplant kidney PME/Pi ratio and the development of ATN. However, the predictive value of a low PME/Pi ratio was too low (36%) to reliably predict development of ATN in individual cases. Extrapolation of the PME/Pi ratio to the time of nephrectomy increased the predictive value to 67%