Tamoxifen-inducible Cre-recombination in articular chondrocytes of adult Col2a1-CreERT2 transgenic mice
- Zhu, M.
- Chen, M.
- Lichtler, A.C.
- O'Keefe, R.J.
- Chen, D.
Publication date
January 2008
DOI Publisher
Osteoarthritis Research Society International. Published by Elsevier Ltd.Abstract
SummaryObjectiveTo determine the specificity and efficiency of the tamoxifen (TM)-induced Cre-recombination in articular chondrocytes of adult Col2a1-CreERT2 transgenic mice.MethodsCol2a1-CreERT2 transgenic mice were bred with Rosa26 reporter mice. Two-week-old Col2a1-CreERT2;R26R mice were administered TM for 5 days and were sacrificed 1 and 6 months after TM induction. X-Gal staining was performed.ResultsEfficient Cre-recombination is achieved in adult articular chondrocytes 1 and 6 months after TM induction.ConclusionOur findings demonstrate that the Col2a1-CreERT2 transgenic mouse model is a valuable tool to target genes specifically expressed in articular chondrocytes in a temporally controlled manner in adult mice
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