AbstractObjective To quantify the expression of genes encoding extracellular matrix (ECM) proteins in human cartilage from normal and osteoarthritic (OA) joints.Design Human cartilage samples were classified as control (CTR) or OA according to clinical evaluation and assessed histologically and biochemically to confirm the diagnosis. mRNAs encoding collagen types I, II and X, aggrecan, versican, osteopontin and osteocalcin were quantified by real-time reverse transcription-PCR assays and normalized to a reference mRNA (GAPDH).ResultsRNA from native cartilage could be reproducibly and efficiently amplified by real-time PCR only if isolated using purification membranes. Primers and fluorescent probes for real-time PCR, endowed with comparable...
Objective: To analyze cartilage gene expression of patients with osteoarthritis (OA) in correlation ...
OBJECTIVE: The aim of this study was to develop high-throughput assays for the analysis of major cho...
Copyright © 2005 Elsevier Inc. All rights reserved.Osteoarthritis (OA) is a common age-related joint...
AbstractObjective To quantify the expression of genes encoding extracellular matrix (ECM) proteins i...
SummaryIntroductionAlthough the extracellular matrix (ECM) is the functional element in articular ca...
AbstractObjective To prepare, sequence and analyse adult human cartilage cDNA libraries to study the...
SummaryObjectiveTo elucidate disease-specific molecular changes in osteoarthritis (OA) by analyzing ...
OBJECTIVE: To understand changes in gene expression levels that occur during osteoarthritic (OA) car...
Background Assessment of gene expression is an important component of osteoarthritis (OA) research,...
<p><b>Copyright information:</b></p><p>Taken from "SOX9 transduction of a human chondrocytic cell li...
The regulation of chondrocytes in osteoarthritic cartilage and the expression of specific gene produ...
SummaryObjectiveTo define for the first time the transcriptomes of normal and end-stage osteoarthrit...
SummaryObjectiveIdentification of genes differentially expressed in mechano-biological pathways in a...
OBJECTIVE: Identify gene expression profiles associated with OA processes in articular cartilage and...
SummaryObjectiveThe aim of this study was to develop high-throughput assays for the analysis of majo...
Objective: To analyze cartilage gene expression of patients with osteoarthritis (OA) in correlation ...
OBJECTIVE: The aim of this study was to develop high-throughput assays for the analysis of major cho...
Copyright © 2005 Elsevier Inc. All rights reserved.Osteoarthritis (OA) is a common age-related joint...
AbstractObjective To quantify the expression of genes encoding extracellular matrix (ECM) proteins i...
SummaryIntroductionAlthough the extracellular matrix (ECM) is the functional element in articular ca...
AbstractObjective To prepare, sequence and analyse adult human cartilage cDNA libraries to study the...
SummaryObjectiveTo elucidate disease-specific molecular changes in osteoarthritis (OA) by analyzing ...
OBJECTIVE: To understand changes in gene expression levels that occur during osteoarthritic (OA) car...
Background Assessment of gene expression is an important component of osteoarthritis (OA) research,...
<p><b>Copyright information:</b></p><p>Taken from "SOX9 transduction of a human chondrocytic cell li...
The regulation of chondrocytes in osteoarthritic cartilage and the expression of specific gene produ...
SummaryObjectiveTo define for the first time the transcriptomes of normal and end-stage osteoarthrit...
SummaryObjectiveIdentification of genes differentially expressed in mechano-biological pathways in a...
OBJECTIVE: Identify gene expression profiles associated with OA processes in articular cartilage and...
SummaryObjectiveThe aim of this study was to develop high-throughput assays for the analysis of majo...
Objective: To analyze cartilage gene expression of patients with osteoarthritis (OA) in correlation ...
OBJECTIVE: The aim of this study was to develop high-throughput assays for the analysis of major cho...
Copyright © 2005 Elsevier Inc. All rights reserved.Osteoarthritis (OA) is a common age-related joint...