Fluorescence properties of the Ca2+Mg2+-ATPase protein of sarcoplasmic reticulum labeled with 7-chloro-4-nitrobenzo-2-oxa-1,3- diazole

Bailin, Gary
Huang, John R.

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Publication date

January 1990

DOI

10.1016/0014-5793(90)80021-A

Publisher

Published by Elsevier B.V.

Abstract

AbstractThe fluorescence intensity of the Ca2+,Mg2+-ATPase protein of rabbit skeletal sarcoplasmic reticulum that incorporated about 2 mol of 7-chloro-4-nitrobenzo-2-oxa-1, 3-diazole (NBD-Cl) was enhanced at high MgATP concentrations with or without 50 μM calcium. The observed enhancement indicates that the fluorophore, NBD-Cl, can detect conformational changes in the ATPase protein