The γ-subunit of ATP synthase from spinach chloroplasts Primary structure deduced from the cloned cDNA sequence

AbstractcDNA clones encoding the γ-subunit of chloroplast ATP synthase were isolated from a spinach library using synthetic oligonucleotide probes. The predicted amino acid sequence indicated that the mature chloroplast γ-subunit consists of 323 amino acid residues and is highly homologous (55% identical residues) with the sequence of the cyanobacterial subunit. The positions of the four cysteine residues were identified. The carboxyl-terminal region of the choloroplast γ-subunit is highly homologous with those of the γ-subunits from six other sources (bacteria and mitochondria) sequenced thus far