It has been hypothesized that nonspecific reversible binding of cytoskeletal proteins to lipids in cells may guide their binding to integral membrane anchor proteins. In a model system, we measured desorption rates k(off) (off-rates) of the erythrocyte cytoskeletal proteins spectrin and protein 4.1 labeled with carboxyfluorescein (CF), at two different compositions of planar phospholipid membranes (supported on glass), using the total internal reflection/fluorescence recovery after photobleaching (TIR/FRAP) technique. The lipid membranes consisted of either pure phosphatidylcholine (PC) or a 3:1 mixture of PC with phosphatidylserine (PS). In general, the off-rates were not single exponentials and were fit to a combination of fast, slow, and...
Key enzymes involved in transmembrane signaling and lipid metabolism (e.g., protein kinase C and pho...
AbstractThe mechanism by which cross-linked glycosylphosphatidylinositol (GPI)-anchored proteins are...
The nonspecific binding of proteins to various biological and non-biological surfaces has limited th...
Non-specific reversible binding of cytoskeletal proteins to lipids may help guide those proteins to ...
It has been hypothesized that nonspecific reversible binding of cytoskeletal proteins to lipids in c...
Reversible binding among components of the cellular submembrane cytoskeleton and reversible binding ...
Protein 4.1 is a major component of the membrane skeleton, mediating the spectrin-actin interaction ...
The localization of peripheral proteins to specific sites of cell membranes is a pivotal step in man...
AbstractThe binding of human erythrocyte spectrin to large unilamellar vesicles (LUVET) formed by th...
AbstractHuman erythrocyte spectrin was labelled with the probe 5,5′-disulfato-1-(6-hexanoic acid N-h...
Interactions between plasma membrane-associated proteins on interacting cells are critical for many ...
Key enzymes. involved in transmembrane signaling and lipid metabolism (e.g., protein kinase C and ph...
1.(1) The interaction of synthetic and natural phospholipids with spectrin, purified from human eryt...
AbstractThe method of fluorescence resonance energy transfer (FRET) has been employed to monitor cyt...
Transient recruitment of proteins to membranes is a fundamental mechanism by which the cell exerts s...
Key enzymes involved in transmembrane signaling and lipid metabolism (e.g., protein kinase C and pho...
AbstractThe mechanism by which cross-linked glycosylphosphatidylinositol (GPI)-anchored proteins are...
The nonspecific binding of proteins to various biological and non-biological surfaces has limited th...
Non-specific reversible binding of cytoskeletal proteins to lipids may help guide those proteins to ...
It has been hypothesized that nonspecific reversible binding of cytoskeletal proteins to lipids in c...
Reversible binding among components of the cellular submembrane cytoskeleton and reversible binding ...
Protein 4.1 is a major component of the membrane skeleton, mediating the spectrin-actin interaction ...
The localization of peripheral proteins to specific sites of cell membranes is a pivotal step in man...
AbstractThe binding of human erythrocyte spectrin to large unilamellar vesicles (LUVET) formed by th...
AbstractHuman erythrocyte spectrin was labelled with the probe 5,5′-disulfato-1-(6-hexanoic acid N-h...
Interactions between plasma membrane-associated proteins on interacting cells are critical for many ...
Key enzymes. involved in transmembrane signaling and lipid metabolism (e.g., protein kinase C and ph...
1.(1) The interaction of synthetic and natural phospholipids with spectrin, purified from human eryt...
AbstractThe method of fluorescence resonance energy transfer (FRET) has been employed to monitor cyt...
Transient recruitment of proteins to membranes is a fundamental mechanism by which the cell exerts s...
Key enzymes involved in transmembrane signaling and lipid metabolism (e.g., protein kinase C and pho...
AbstractThe mechanism by which cross-linked glycosylphosphatidylinositol (GPI)-anchored proteins are...
The nonspecific binding of proteins to various biological and non-biological surfaces has limited th...