Detection Of Human Papilloma Virus (Type 16 And 18) In Cytological Samples In Patients With Lung Cancer. An Evaluation By Chromogenic In Situ Hybridization Technique

BackgroundLung cancer is a common neoplasm. Some studies suggested that HPV may play an etiologic role in bronchial carcinogenesis and possibility of a latent HPV infection as a cocarcinogen cannot be excluded. HPV (high risk types) 16 &18 may affect the cell cycle and inhibit apoptosis, allowing uncontrolled cell division.Aims of study1. To use bronchial wash as a non invasive procedure to determine the presence of DNA of high risk types (16 &18 )of HPV in patients with lung cancer using in situ hybridization technique. 2. To Find the association of the score or intensity of viral in situ hybridization signals with histopathological types.Patients, Materials and methodsA prospective study, whereby bronchial wash of 50 patients diagnosed cytologicaly as having lung cancer together with samples from 30 patient having chronic illness as control.ResultsThe most affected age group was 66–70 years (28% of the cases). Males were affected more frequently than females (64.0%). Regarding HPV16 & 18 and histological types of lung cancer the commonest effected type was squamouse cell carcinoma showed positive signals in 32% and 28% for HPV16&HPV18 respectively. Percentage score and intensity score: Lung cancer samples showed low intensity signals for human papilloma virus16 in 63.6% of samples and 44.5% for human papilloma virus 18.ConclusionsHuman papilloma virus 16 and 18 was detected in cytological bronchial samples of patients with lung cancer in relatively high percentag