T7 endonuclease I is a nuclease that is selective for the structure of the four-way DNA junction. The active site is similar to those of a number of restriction enzymes. We have solved the crystal structure of endonuclease I with a wild-type active site. Diffusion of manganese ions into the crystal revealed two peaks of electron density per active site, defining two metal ion-binding sites. Site 1 is fully occupied, and the manganese ion is coordinated by the carboxylate groups of Asp55 and Glu65, and the main chain carbonyl of Thr66. Site 2 is partially occupied, and the metal ion has a single protein ligand, the remaining carboxylate oxygen atom of Asp55. Isothermal titration calorimetry showed the sequential exothermic binding of two man...
<p><b>A:</b> Electron density for rUMP in pH1N1 PA endonuclease. Manganese ions are pink spheres, co...
Bacteriophages and large dsDNA viruses encode sophisticated machinery to translocate their DNA into ...
Restriction endonucleases of the PD...D/EXK family need Mg2+ for DNA cleavage. Whereas Mg2+ (or Mn2+...
Endonuclease I of bacteriophage T7 is a DNA junction-resolving enzyme. We have previously used cryst...
The chemical mechanism of phosphodiester bond hydrolysis catalyzed by a junction-resolving enzyme ha...
Endonuclease I is a junction-resolving enzyme encoded by bacteriophage T7, that selectively binds an...
ABSTRACT: Apurinic/apyrimidinic endonuclease I (APE1) is an essential base excision repair enzyme th...
ABSTRACT: The LAGLIDADG homing endonucleases include free-standing homodimers, pseudosymmetric monom...
We have solved the crystal structure of the Holliday junction resolving enzyme T7 endonuclease I at ...
A majority of enzymes show a high degree of specificity toward a particular metal ion in their catal...
Controversy surrounds the metal-dependent mechanism of H-N-H endonucleases, enzymes involved in a va...
A majority of enzymes show a high degree of specificity toward a particular metal ion in their catal...
The choreography of restriction endonuclease catalysis is a long-standing paradigm in molecular biol...
A typical feature of type II restriction endonucleases (REases) is their obligate sequence specifici...
A typical feature of type II restriction endonucleases (REases) is their obligate sequence specifici...
<p><b>A:</b> Electron density for rUMP in pH1N1 PA endonuclease. Manganese ions are pink spheres, co...
Bacteriophages and large dsDNA viruses encode sophisticated machinery to translocate their DNA into ...
Restriction endonucleases of the PD...D/EXK family need Mg2+ for DNA cleavage. Whereas Mg2+ (or Mn2+...
Endonuclease I of bacteriophage T7 is a DNA junction-resolving enzyme. We have previously used cryst...
The chemical mechanism of phosphodiester bond hydrolysis catalyzed by a junction-resolving enzyme ha...
Endonuclease I is a junction-resolving enzyme encoded by bacteriophage T7, that selectively binds an...
ABSTRACT: Apurinic/apyrimidinic endonuclease I (APE1) is an essential base excision repair enzyme th...
ABSTRACT: The LAGLIDADG homing endonucleases include free-standing homodimers, pseudosymmetric monom...
We have solved the crystal structure of the Holliday junction resolving enzyme T7 endonuclease I at ...
A majority of enzymes show a high degree of specificity toward a particular metal ion in their catal...
Controversy surrounds the metal-dependent mechanism of H-N-H endonucleases, enzymes involved in a va...
A majority of enzymes show a high degree of specificity toward a particular metal ion in their catal...
The choreography of restriction endonuclease catalysis is a long-standing paradigm in molecular biol...
A typical feature of type II restriction endonucleases (REases) is their obligate sequence specifici...
A typical feature of type II restriction endonucleases (REases) is their obligate sequence specifici...
<p><b>A:</b> Electron density for rUMP in pH1N1 PA endonuclease. Manganese ions are pink spheres, co...
Bacteriophages and large dsDNA viruses encode sophisticated machinery to translocate their DNA into ...
Restriction endonucleases of the PD...D/EXK family need Mg2+ for DNA cleavage. Whereas Mg2+ (or Mn2+...