) Synthesis and Degradation Enzymes during Differentiation of Immortalized Urothelial Cells from Patients with Interstitial Cystitis/Painful Bladder Syndrome

synthesis and degradation enzymes in urothelial cells during differentiation. synthase (PGES) and 15-hydroxyprostaglandin dehydrogenase (PGDH) in human urothelial cells and in immortalized urothelial cells isolated from the bladders of IC/PBS patients or normal subjects during stratification and differentiation produced by increased calcium and fetal bovine serum (Ca/FBS) in the culture medium for 1, 3 and 7 days. release in response to tryptase under any of the experimental conditions studied. release is compromised during stratification and differentiation in IC/PBS urothelium and may contribute to impaired barrier function