Nano-to-Micro Scale Dynamics of P-Selectin Detachment from Leukocyte Interfaces. III. Numerical Simulation of Tethering under Flow
- King, Michael R.
- Heinrich, Volkmar
- Evans, Evan
- Hammer, Daniel A.
DOIAbstract
AbstractTransient capture of cells or model microspheres from flow over substrates sparsely coated with adhesive ligands has provided significant insight into the unbinding kinetics of leukocyte:endothelium adhesion complexes under external force. Whenever a cell is stopped by a point attachment, the full hydrodynamic load is applied to the adhesion site within an exceptionally short time—less than the reciprocal of the hydrodynamic shear rate (e.g., typically <0.01s). The decay in numbers of cells or beads that remain attached to a surface has been used as a measure of the kinetics of molecular bond dissociation under constant force, revealing a modest increase in detachment rate at growing applied shear stresses. On the other hand, when d...
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