Outer Balloon Ligation Increases Success Rate of Ischemia-Reperfusion Injury Model in Mice

.The purpose of this study was to describe a new method (outer balloon ligation) to induce myocardial I/R injury in mice.Ninety-nine male C57BL/6J mice were randomly divided into three groups: sham group, classic method group (I/R-C) and the new method group (I/R-N). The surgical procedure and recovery time were recorded. The levels of TNF-α, IL-6, cTnT and LDH were detected by ELISA kits. Hematoxylin-eosin staining was applied to assess neutrophil infiltration. Moreover, surgical survival, myocardial infarction areas, and cardiac function measurements were also recorded.