AbstractLooping and cleavage of single DNA molecules by the two-site restriction endonuclease Sau3AI were measured with optical tweezers. A DNA template containing many recognition sites was used, permitting loop sizes from ∼10 to 10,000 basepairs. At high enzyme concentration, cleavage events were detected within 5s and nearly all molecules were cleaved within 5min. Activity decreased ∼10-fold as the DNA tension was increased from 0.03 to 0.7pN. Substituting Ca2+ for Mg2+ blocked cleavage, permitting measurement of stable loops. At low tension, the initial rates of cleavage and looping were similar (∼0.025s−1 at 0.1pN), suggesting that looping is rate limiting. Short loops formed more rapidly than long loops. The optimum size decreased fro...
AbstractGene transcription by the enzyme RNA polymerase is tightly regulated. In many cases, such as...
Many proteins that interact with DNA perform or enhance their specific functions by binding simultan...
Structural deformation of DNA has a central role in many biological processes. It occurs, for exampl...
AbstractLooping and cleavage of single DNA molecules by the two-site restriction endonuclease Sau3AI...
The force-measuring optical tweezers has afforded scientists unprecedented insights into many of the...
AbstractProtein-mediated DNA looping is important in a variety of biological processes, including ge...
DNA loops form when a single protein or protein complex binds to multiple sites on the same DNA mole...
The interior of a cell is a crowded and constantly fluctuating environment, where DNA and other biom...
The SfiI endonuclease is a prototype for DNA looping. It binds two copies of its recognition sequenc...
Type II restriction endonucleases protect bacteria against phage infections by cleaving recognition ...
Many restriction enzymes require binding of two copies of a recognition sequence for DNA cleavage, t...
Protein-induced DNA looping is crucial for many genetic processes such as transcription, gene regula...
The tethered particle motion (TPM) allows the direct detection of activity of a variety of biomolecu...
Mechanical properties of DNA are known to play a significant role in several biological processes li...
Many restriction enzymes require binding of two copies of a recognition sequence for DNA cleavage, t...
AbstractGene transcription by the enzyme RNA polymerase is tightly regulated. In many cases, such as...
Many proteins that interact with DNA perform or enhance their specific functions by binding simultan...
Structural deformation of DNA has a central role in many biological processes. It occurs, for exampl...
AbstractLooping and cleavage of single DNA molecules by the two-site restriction endonuclease Sau3AI...
The force-measuring optical tweezers has afforded scientists unprecedented insights into many of the...
AbstractProtein-mediated DNA looping is important in a variety of biological processes, including ge...
DNA loops form when a single protein or protein complex binds to multiple sites on the same DNA mole...
The interior of a cell is a crowded and constantly fluctuating environment, where DNA and other biom...
The SfiI endonuclease is a prototype for DNA looping. It binds two copies of its recognition sequenc...
Type II restriction endonucleases protect bacteria against phage infections by cleaving recognition ...
Many restriction enzymes require binding of two copies of a recognition sequence for DNA cleavage, t...
Protein-induced DNA looping is crucial for many genetic processes such as transcription, gene regula...
The tethered particle motion (TPM) allows the direct detection of activity of a variety of biomolecu...
Mechanical properties of DNA are known to play a significant role in several biological processes li...
Many restriction enzymes require binding of two copies of a recognition sequence for DNA cleavage, t...
AbstractGene transcription by the enzyme RNA polymerase is tightly regulated. In many cases, such as...
Many proteins that interact with DNA perform or enhance their specific functions by binding simultan...
Structural deformation of DNA has a central role in many biological processes. It occurs, for exampl...