Characterization of the Estradiol-Binding Site Structure of Human Protein Disulfide Isomerase (PDI)

-binding site is still unclear at present, which is the focus of this study. and His256 of PDI is critical for the binding interaction. This binding model was jointly confirmed by a series of detailed experiments, including site-directed mutagenesis of the His256 residue coupled with selective modifications of the ligand structures to alter the binding interaction.-binding site structure of human PDI determined here offers structural insights which may aid in the rational design of novel PDI inhibitors