HIV Drug Resistance Mutations in Proviral DNA from a Community Treatment Program

Derache Anne
Shin Hyoung-Shik
Balamane Maya
White Elizabeth
Israelski Dennis
Klausner Jeffrey D.
Freeman Alexandra H.
Katzenstein David

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Publication date

January 2015

DOI

10.1371/journal.pone.0117430

Publisher

Public Library of Science

Abstract

Drug resistance mutations archived in resting memory CD4+ cells may persist despite suppression of HIV RNA to <50 copies/ml. We sequenced pol gene from proviral DNA among viremic and suppressed patients to identify drug resistance mutations..Among 120 subjects, 81% were on antiretroviral therapy and had been treated for a median time of 7 years. Thirty-two viremic patients showed concordant RNA and DNA genotypes (84%); the discordant profiles were mainly observed in patients with low-level viremia. Among suppressed patients, 21 had drug resistance mutations in proviral DNA (26%) with potential resistance to one, two or three ARV classes in 16, 4 and 1 samples respectively.The high level of genotype concordance between DNA and RNA in viremic...