Transcriptome Sequencing, and a Comparison with Amplified Fragment Length Polymorphism (AFLP) Markers
- Smee Melanie R.
- Pauchet Yannick
- Wilkinson Paul
- Wee Brian
- Singer Michael C.
- ffrench-Constant Richard H.
- Hodgson David J.
- Mikheyev Alexander S.
DOIAbstract
assembled transcriptome sequence, and genotyping the microsatellites found there using an economic method of fluorescently labelling primers. outlier approach to show that these markers are likely selectively neutral. Furthermore, using a set of 128 individuals from 11 populations, we demonstrate consistency in population differentiation estimates with previously developed amplified fragment length polymorphism (AFLP) markers (r = 0.68, p<0.001). transcriptional sequencing for future studies of population structure and gene flow that are desperately needed for declining species across fragmented landscapes
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