Inositol pyrophosphates are a recently characterized cell signalling molecules responsible for the pyrophosphorylation of protein substrates. Though likely involved in a wide range of cellular functions, the study of inositol pyrophosphates has suffered from a lack of readily available methods for their analysis.We describe a novel, sensitive and rapid polyacrylamide gel electrophoresis (PAGE)-based method for the analysis of inositol pyrophosphates. Using 4′,6-diamidino-2-phenylindole (DAPI) and Toluidine Blue we demonstrate the unequivocal detection of various inositol pyrophosphate species.The use of the PAGE-based method reveals the likely underestimation of inositol pyrophosphates and their signalling contribution in cells when measure...
Inositol-based signaling molecules are central eukaryotic messengers and include the highly phosphor...
open access articleInositol pyrophosphates are important biomolecules associated with apoptosis, cel...
Inositol pyrophosphates (PP-IPs) such as 5-diphosphoinositol pentakisphosphate (5-IP7) are inositol ...
BACKGROUND:Inositol pyrophosphates are a recently characterized cell signalling molecules responsibl...
Background: Inositol pyrophosphates are a recently characterized cell signalling molecules responsib...
The inositol polyphosphate family of small, cytosolic molecules has a prominent place in the field o...
The social amoeba Dictyostelium discoideum was instrumental in the discovery and early characterizat...
<div><p>The social amoeba <i>Dictyostelium discoideum</i> was instrumental in the discovery and earl...
The social amoeba Dictyostelium discoideum was instrumental in the discovery and early characterizat...
Inositolpyrophosphate (PP-InsPs) sind eine wichtige Gruppe eukaryotischer Botenstoffe, die mit versc...
Inositol phosphates are naturally occurring compounds that regulate diverse cellular processes inclu...
The HCT116 cell line, which has a pseudo-diploid karotype, is a popular model in the fields of cance...
SummaryEukaryotic cells produce a family of diverse inositol polyphosphates (IPs) containing pyropho...
Pharmacological tools-'chemical probes'-that intervene in cell signaling cascades are important for ...
Genetic ablation of inositol pyrophosphate synthesis has established the fundamental importance of t...
Inositol-based signaling molecules are central eukaryotic messengers and include the highly phosphor...
open access articleInositol pyrophosphates are important biomolecules associated with apoptosis, cel...
Inositol pyrophosphates (PP-IPs) such as 5-diphosphoinositol pentakisphosphate (5-IP7) are inositol ...
BACKGROUND:Inositol pyrophosphates are a recently characterized cell signalling molecules responsibl...
Background: Inositol pyrophosphates are a recently characterized cell signalling molecules responsib...
The inositol polyphosphate family of small, cytosolic molecules has a prominent place in the field o...
The social amoeba Dictyostelium discoideum was instrumental in the discovery and early characterizat...
<div><p>The social amoeba <i>Dictyostelium discoideum</i> was instrumental in the discovery and earl...
The social amoeba Dictyostelium discoideum was instrumental in the discovery and early characterizat...
Inositolpyrophosphate (PP-InsPs) sind eine wichtige Gruppe eukaryotischer Botenstoffe, die mit versc...
Inositol phosphates are naturally occurring compounds that regulate diverse cellular processes inclu...
The HCT116 cell line, which has a pseudo-diploid karotype, is a popular model in the fields of cance...
SummaryEukaryotic cells produce a family of diverse inositol polyphosphates (IPs) containing pyropho...
Pharmacological tools-'chemical probes'-that intervene in cell signaling cascades are important for ...
Genetic ablation of inositol pyrophosphate synthesis has established the fundamental importance of t...
Inositol-based signaling molecules are central eukaryotic messengers and include the highly phosphor...
open access articleInositol pyrophosphates are important biomolecules associated with apoptosis, cel...
Inositol pyrophosphates (PP-IPs) such as 5-diphosphoinositol pentakisphosphate (5-IP7) are inositol ...