High Diversity and Variability in the Vaginal Microbiome in Women following Preterm Premature Rupture of Membranes (PPROM): A Prospective Cohort Study

To characterize the vaginal microbiota of women following preterm premature rupture of membranes (PPROM), and determine if microbiome composition predicts latency duration and perinatal outcomes.A prospective cohort studyCanada weeks gestational age (GA).60 universal target, were generated from vaginal samples at time of presentation with PPROM, weekly thereafter, and at delivery.Vaginal microbiome composition, latency duration, gestational age at delivery, perinatal outcomes. negative women. type I were ubiquitous. The presence of Mollicutes in the vaginal microbiome was associated with lower GA at delivery. The microbiome was remarkably unstable during the latency period