Mice Deficient in GEM GTPase Show Abnormal Glucose Homeostasis Due to Defects in Beta-Cell Calcium Handling
- Gunton Jenny E.
- Sisavanh Mary
- Stokes Rebecca A.
- Satin Jon
- Satin Leslie S.
- Zhang Min
- Liu Sue M.
- Cai Weikang
- Cheng Kim
- Cooney Gregory J.
- Laybutt D. Ross
- So Trina
- Molero Juan-Carlos
- Grey Shane T.
- Andres Douglas A.
- Rolph Michael S.
- Mackay Charles R.
DOIAbstract
.Gem-deficient mice were generated and their metabolic phenotype characterised by in vivo testing of glucose tolerance, insulin tolerance and insulin secretion. Calcium flux was measured in isolated islets.Gem-deficient mice were glucose intolerant and had impaired glucose stimulated insulin secretion. Furthermore, the islets of Gem-deficient mice exhibited decreased free calcium responses to glucose and the calcium oscillations seen upon glucose stimulation were smaller in amplitude and had a reduced frequency.These results suggest that Gem plays an important role in normal beta-cell function by regulation of calcium signalling
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