Anti-loxoscelic horse serum produced against a recombinant dermonecrotic protein of Brazilian Loxosceles intermedia spider neutralize lethal effects of Loxosceles laeta venom from Peru
- Duarte, C.G.
- Bonilla, C.
- Guimarães, G.
- Machado de Avila, R.A.
- Mendes, T.M.
- Silva, W.
- Tintaya, B.
- Yarleque, A.
- Chávez-Olórtegui, C.
DOIAbstract
AbstractIn this work, an anti-loxoscelic serum was produced by immunizing horses with a recombinant dermonecrotic protein from Loxosceles intermedia (rLiD1). Anti-rLiD1 antibodies were able to recognize different species of Loxosceles venoms by Western Blot and ELISA. The efficacy of anti-rLiD1 serum against the toxic effects of Loxosceles laeta (Peru) venom was tested, showing that anti-rLiD1 serum can neutralize those effects. This study confirms that recombinant proteins can be good candidates to replace crude venoms for antivenom production
Add to ORKG