cDNA cloning and chromosomal localization of the human α-adrenergic receptor kinase

AbstractThe β-adrenergic receptor kinase (βARK) mediates agonist-dependent phosphorylation of the β2-adrenergic and related G protein-coupled receptors. A cDNA encoding bovine βARK has recently been isolated. In this work we have isolated a cDNA encoding human βARK from a retinal cDNA library. The cDNA encodes a protein of 689 amino acids with an overall 98.0% amino acid and 92.5% nucleotide identity with bovine βARK. Chromosomal location of the human βARK gene was determined by correlating the presence of the βARK locus with retention of a specific human chromosome in a rodent-human hybrid panel. This analysis revealed that the human βARK locus segregated with the long arm of chromosome 11, centromeric to 11q13