Modulation of bovine articular chondrocyte gene expression in vitro by oxygen tension
- Grimshaw, M.J.
- Mason, R.M.
DOIAbstract
AbstractObjective Adult articular cartilage is a physiologically hypoxic tissue with a proposed gradient of oxygen tension ranging from <10% oxygen at the cartilage surface to <1% in the deepest layers. This gradient may be disturbed during diseases of the joint, for example in rheumatoid arthritis when synovial fluid pO2falls. We investigated whether changes in oxygen tension modulate gene expression in articular chondrocytes.Design Bovine articular chondrocytes were cultured in alginate beads in medium maintained at <0.1, 5, 10 or 20% oxygen. A modified RNA arbitrarily primed polymerase chain reaction (RAP-PCR) technique was used to identify several genes whose mRNA abundance in articular chondrocytes was dependent upon oxygen tension. No...
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