SummaryCompared to globular proteins, RNAs with complex 3D folds are characterized by poorly differentiated molecular surfaces dominated by backbone phosphates, sparse tertiary contacts stabilizing global architecture, and conformational flexibility. The resulting generally poor order of crystals of large RNAs and their complexes frequently hampers crystallographic structure determination. We describe and rationalize a postcrystallization treatment strategy that exploits the importance of solvation and counterions for RNA folding. Replacement of Li+ and Mg2+ needed for growth of crystals of a tRNA-riboswitch-protein complex with Sr2+, coupled with dehydration, dramatically improved the resolution limit (8.5–3.2 Å) and data quality, enabling...
Recent discovery of structured RNAs such as ribozymes and riboswitches shows that there is still muc...
Crystallizing RNA has been an imperative and challenging task in the world of RNA research. Assistiv...
We present a simple approach to locate sites that undergo conformational changes upon crystallizatio...
SummaryCompared to globular proteins, RNAs with complex 3D folds are characterized by poorly differe...
X-ray crystallography remains a powerful method to gain atomistic insights into the catalytic and re...
AbstractBackground: The structure of P4-P6, a 160 nucleotide domain of the self-splicing Tetrahymena...
This is the final version of the following article : New crystallization strategies to fast-feed str...
Crystallography is a major technique for determining large RNA structures. Obtaining diffraction-qua...
Although the regulatory, signaling, and catalytic functions of RNA are comparable to their protein c...
SummaryX-ray crystallography of biologically important RNA molecules has been hampered by technical ...
Preparation of suitably large and wellâ ordered single crystals is usually the rateâ limiting step...
The three-dimensional structures of RNA enzymes form catalytic centers that include specific substra...
Crystallizing RNA has been an imperative and challenging task in the world of RNA research. Assistiv...
A high quality single crystallization of protein is indispensable for X-ray crystallography. However...
Preliminary phases were determined by the application of the isomorphous replacement method at low a...
Recent discovery of structured RNAs such as ribozymes and riboswitches shows that there is still muc...
Crystallizing RNA has been an imperative and challenging task in the world of RNA research. Assistiv...
We present a simple approach to locate sites that undergo conformational changes upon crystallizatio...
SummaryCompared to globular proteins, RNAs with complex 3D folds are characterized by poorly differe...
X-ray crystallography remains a powerful method to gain atomistic insights into the catalytic and re...
AbstractBackground: The structure of P4-P6, a 160 nucleotide domain of the self-splicing Tetrahymena...
This is the final version of the following article : New crystallization strategies to fast-feed str...
Crystallography is a major technique for determining large RNA structures. Obtaining diffraction-qua...
Although the regulatory, signaling, and catalytic functions of RNA are comparable to their protein c...
SummaryX-ray crystallography of biologically important RNA molecules has been hampered by technical ...
Preparation of suitably large and wellâ ordered single crystals is usually the rateâ limiting step...
The three-dimensional structures of RNA enzymes form catalytic centers that include specific substra...
Crystallizing RNA has been an imperative and challenging task in the world of RNA research. Assistiv...
A high quality single crystallization of protein is indispensable for X-ray crystallography. However...
Preliminary phases were determined by the application of the isomorphous replacement method at low a...
Recent discovery of structured RNAs such as ribozymes and riboswitches shows that there is still muc...
Crystallizing RNA has been an imperative and challenging task in the world of RNA research. Assistiv...
We present a simple approach to locate sites that undergo conformational changes upon crystallizatio...