SummaryProtein levels have been shown to vary substantially between individual cells in clonal populations. In prokaryotes, the contribution to such fluctuations from the inherent randomness of gene expression has largely been attributed to having just a few transcripts of the corresponding mRNAs. By contrast, eukaryotic studies tend to emphasize chromatin remodeling and burst-like transcription. Here, we study single-cell transcription in Escherichia coli by measuring mRNA levels in individual living cells. The results directly demonstrate transcriptional bursting, similar to that indirectly inferred for eukaryotes. We also measure mRNA partitioning at cell division and correlate mRNA and protein levels in single cells. Partitioning is app...
The blueprint of a living cell is inscribed in its DNA. A region of DNA encoding a protein is called...
Thesis: Ph. D., Massachusetts Institute of Technology, Department of Electrical Engineering and Comp...
Single-cell experiments reveal considerable fluctuations in the expression of both mRNA and protein....
Protein and messenger RNA (mRNA) copy numbers vary from cell to cell in isogenic bacterial populatio...
Transcriptional stochasticity can be measured by counting the number of mRNA molecules per cell. Cel...
The phenotypic characteristics of living organisms are shaped by the interactions of the genotype wi...
A long-standing question in quantitative biology is the relationship between mRNA and protein levels...
Until recently, transcription could only be observed by measuring mRNA production of cell population...
One of the qualities that allow bacterial cells to survive in diverse, fluctuating environments is ph...
The process of gene expression has two seemingly opposite characteristics: it is highly regulated on...
In all organisms, cellular functions, such as growth and differentiation, are coordinated by gene ne...
The quantitative relation between transcription factor concentrations and the rate of protein produc...
In prokaryotes, transcription and translation are dynamically coupled, as the latter starts before t...
\u3cp\u3eThe level of a given mRNA or protein exhibits significant variations from cell-to-cell acro...
Inside individual cells, expression of genes is inherently stochastic and manifests as cell-to-cell ...
The blueprint of a living cell is inscribed in its DNA. A region of DNA encoding a protein is called...
Thesis: Ph. D., Massachusetts Institute of Technology, Department of Electrical Engineering and Comp...
Single-cell experiments reveal considerable fluctuations in the expression of both mRNA and protein....
Protein and messenger RNA (mRNA) copy numbers vary from cell to cell in isogenic bacterial populatio...
Transcriptional stochasticity can be measured by counting the number of mRNA molecules per cell. Cel...
The phenotypic characteristics of living organisms are shaped by the interactions of the genotype wi...
A long-standing question in quantitative biology is the relationship between mRNA and protein levels...
Until recently, transcription could only be observed by measuring mRNA production of cell population...
One of the qualities that allow bacterial cells to survive in diverse, fluctuating environments is ph...
The process of gene expression has two seemingly opposite characteristics: it is highly regulated on...
In all organisms, cellular functions, such as growth and differentiation, are coordinated by gene ne...
The quantitative relation between transcription factor concentrations and the rate of protein produc...
In prokaryotes, transcription and translation are dynamically coupled, as the latter starts before t...
\u3cp\u3eThe level of a given mRNA or protein exhibits significant variations from cell-to-cell acro...
Inside individual cells, expression of genes is inherently stochastic and manifests as cell-to-cell ...
The blueprint of a living cell is inscribed in its DNA. A region of DNA encoding a protein is called...
Thesis: Ph. D., Massachusetts Institute of Technology, Department of Electrical Engineering and Comp...
Single-cell experiments reveal considerable fluctuations in the expression of both mRNA and protein....