Endovascular biopsy: Strategy for analyzing gene expression profiles of individual endothelial cells obtained from human vessels

Sun, Zhengda
Lawson, Devon A.
Sinclair, Elizabeth
Wang, Chih-Yang
Lai, Ming-Derg
Hetts, Steven W.
Higashida, Randall T.
Dowd, Christopher F.
Halbach, Van V.
Werb, Zena
Su, Hua
Cooke, Daniel L.

Open link

Publication date

September 2015

DOI

10.1016/j.btre.2015.07.001

Publisher

The Authors. Published by Elsevier B.V.

Abstract

AbstractPurposeTo develop a strategy of achieving targeted collection of endothelial cells (ECs) by endovascular methods and analyzing the gene expression profiles of collected single ECs.Methods and results134 ECs and 37 leukocytes were collected from four patients' intra-iliac artery endovascular guide wires by fluorescence activated cell sorting (FACS) and analyzed by single-cell quantitative RT-PCR for expression profile of 48 genes. Compared to CD45+ leukocytes, the ECs expressed higher levels (p<0.05) of EC surface markers used on FACS and other EC related genes. The gene expression profile showed that these isolated ECs fell into two clusters, A and B, that differentially expressed 19 genes related to angiogenesis, inflammation and e...