A murine monoclonal antibody (ECS-1) was prepared from BALB/c mice immunized with trypsinized cultured human foreskin keratinocytes. The antibody showed a pattern suggestive of intercellular staining on the nucleated layers of normal human epidermis, adult palm, mouse lip epidermis, and cultured human keratinocytes. ECS-1 stained human fetal skin by 9 weeks estimated gestational age. ECS-1 reacted with a 35 kD protein extracted from neonatal foreskin epidermis and cultured human keratinocytes. The protein required Nonidet P-40 or sodium dodecyl sulfate and mercaptoethanol for solubilization. ECS-1 induced epidermal cell detachment which was enhanced by complement. ECS- 1 shares characteristics with human pemphigus antibodies
Cell isolation and culture. Human primary keratinocytes, COS-1 cells and THP-1 monocytes were cultur...
In order to determine the source of epidermolysis bullosa acquisita (EBA) antigen, we studied its sy...
A monoclonal antikeratin antibody, EKH4, was produced from a hybridoma cell line which was establish...
A murine monoclonal antibody (ECS-1) was prepared from BALB/c mice immunized with trypsinized cultur...
The pathogenic effects of pemphigus vulgaris (PV) antibodies on epidermal cells can be demonstrated ...
International audienceThe therapeutic potential of monoclonal antibodies for treating a variety of s...
A unique monoclonal antibody was obtained by immunizing mice with complement-inactivated fetal bovin...
Somatic cell hybrids were established from the mouse myeloma, P3×63Ag8.653 cells, and the spleen cel...
A monoclonal antibody directed to a species-specific determinant of human epidermal growth factor (h...
Using epidermal cells from psoriatic plaques as the immunogen, an IgG1 murine monoclonal antibody, V...
In studying the mechanisms that regulate the growth and differentiation of the human epidermis, it w...
Antibodies in the sera of patients with the disease bullous pemphigoid define a normal component of ...
Antibodies raised in rabbits to purified cornified envelopes (CEs) of cultured human keratinocytes r...
A monoclonal antibody against keratins (KL1) from normal human stratum corneum was obtained using hy...
Pemphigus vulgaris autoantibodies (PV IgG) promote cell detachment in epidermal cell cultures and ac...
Cell isolation and culture. Human primary keratinocytes, COS-1 cells and THP-1 monocytes were cultur...
In order to determine the source of epidermolysis bullosa acquisita (EBA) antigen, we studied its sy...
A monoclonal antikeratin antibody, EKH4, was produced from a hybridoma cell line which was establish...
A murine monoclonal antibody (ECS-1) was prepared from BALB/c mice immunized with trypsinized cultur...
The pathogenic effects of pemphigus vulgaris (PV) antibodies on epidermal cells can be demonstrated ...
International audienceThe therapeutic potential of monoclonal antibodies for treating a variety of s...
A unique monoclonal antibody was obtained by immunizing mice with complement-inactivated fetal bovin...
Somatic cell hybrids were established from the mouse myeloma, P3×63Ag8.653 cells, and the spleen cel...
A monoclonal antibody directed to a species-specific determinant of human epidermal growth factor (h...
Using epidermal cells from psoriatic plaques as the immunogen, an IgG1 murine monoclonal antibody, V...
In studying the mechanisms that regulate the growth and differentiation of the human epidermis, it w...
Antibodies in the sera of patients with the disease bullous pemphigoid define a normal component of ...
Antibodies raised in rabbits to purified cornified envelopes (CEs) of cultured human keratinocytes r...
A monoclonal antibody against keratins (KL1) from normal human stratum corneum was obtained using hy...
Pemphigus vulgaris autoantibodies (PV IgG) promote cell detachment in epidermal cell cultures and ac...
Cell isolation and culture. Human primary keratinocytes, COS-1 cells and THP-1 monocytes were cultur...
In order to determine the source of epidermolysis bullosa acquisita (EBA) antigen, we studied its sy...
A monoclonal antikeratin antibody, EKH4, was produced from a hybridoma cell line which was establish...