High-throughput epidemiologic typing in clinical microbiology

ABSTRACTMapping, and ultimately preventing, the dissemination of infectious agents is an important topic in public health. Newly developed molecular—microbiological methods have contributed significantly to recent advances in the efficient tracking of the nosocomial and environmental spread of microbial pathogens. Not only has the application of novel technologies led to improved understanding of microbial epidemiology, but the concepts of population structure and dynamics of many of the medically significant microorganisms have advanced significantly also. Currently, genetic identification of microbes is also within the reach of clinical microbiology laboratory professionals including those without specialized technology research interests. This review summarizes the possibilities for high—throughput molecular—microbiological typing in adequately equipped medical microbiology laboratories from both clinical and fundamental research perspectives. First, the development and application of methods for large—scale comparative typing of serially isolated microbial strains are discussed. The outcome of studies employing these methods allows for long—term epidemiologic surveillance of infectious diseases. Second, recent methods enable an almost nucleotide—by-nucleotide genetic comparison of smaller numbers of strains, thereby facilitating the identification of the genetic basis of, for instance, medically relevant microbiological traits. Whereas the first approach provides insights into the dynamic spread of infectious agents, the second provides insights into intragenomic dynamics and genetic functionality. The current state of technology is summarized, and future perspectives are sketched