Molecular characterization of human IgG monoclonal antibodies specific for the major birch pollen allergen Bet v 1. Anti-allergen IgG can enhance the anaphylactic reaction
- Denépoux, Stéphane
- Eibensteiner, Petra B.
- Steinberger, Peter
- Vrtala, Susanne
- Visco, Vincenzo
- Weyer, Anne
- Kraft, Dietrich
- Banchereau, Jacques
- Valenta, Rudolf
- Lebecque, Serge
Publication date
January 2000
DOI Publisher
Federation of European Biochemical Societies. Published by Elsevier B.V.Abstract
AbstractWe report the molecular characterization of five human monoclonal antibodies, BAB1–5 (BAB1: IgG1; BAB4: IgG2; BAB2, 3, 5: IgG4), with specificity for the major birch pollen allergen, Bet v 1. BAB1–5 were obtained after immunotherapy and contained a high degree of somatic mutations indicative of an antigen-driven affinity maturation process. While BAB1 inhibited the binding of patients IgE to Bet v 1, BAB2 increased IgE recognition of Bet v 1, and, even as Escherichia coli-expressed Fab, augmented Bet v 1-induced immediate type skin reactions. The demonstration that IgG antibodies can enhance allergen-induced allergic reactions is likely to explain the unpredictability of specific immunotherapy
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