Novel defensin subfamily from spinach (Spinacia oleracea)

Segura, Ana
Moreno, Manuel
Molina, Antonio
Garcı́a-Olmedo, Francisco

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Publication date

September 1998

DOI

10.1016/S0014-5793(98)01060-6

Publisher

Federation of European Biochemical Societies. Published by Elsevier B.V.

Abstract

AbstractAntimicrobial peptides (So-D1-7) were isolated from a crude cell wall preparation from spinach leaves (Spinacia oleracea cv. Matador) and, judged from their amino acid sequences, six of them (So-D2-7) represented a novel structural subfamily of plant defensins (group IV). Group-IV defensins were also functionally distinct from those of groups I–III. They were active at concentrations <20 μM against Gram-positive (Clavibacter michiganensis) and Gram-negative (Ralstonia solanacearum) bacterial pathogens, as well as against fungi, such as Fusarium culmorum, F. solani, Bipolaris maydis, and Colletotrichum lagenarium. Fungal inhibition occurred without hyphal branching. Group-IV defensins were preferentially distributed in the epidermal ...